(b) The many methods of obtaining differential staining of protoplasm and nucleus depend on the circumstance that, when solutions of certain kinds of methylene blue and eosin are mixed, a third red dye, with a special affinity for chromatin, is formed. They are all modifications of the original Romanowsky method. They are a little uncertain and troublesome to use, their success depending on the purity of the chemicals employed and careful attention to detail. The beginner is advised to become proficient, in the first instance, with the simpler methods given above. Having attained this, he should practise some form of the Romanowsky method, for, besides displaying the intimate structure of the malaria parasite, in consequence of the intense tinting of the nucleus which it effects it greatly facilitates the finding of the smaller forms of the parasite, not always an easy matter, especially in the case of the very minute young subtertian or malignant parasite. Of the three methods given below, Leishman's, everything considered, is the best. Both it and Jenner's method have the advantage of dispensing with preliminary fixing.